dna (fluidigm)
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Structured Review
fluidigm
dna
Dna, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+intercalator/Cell-ID+Intercalator-Ir/10__1038_slash_s41590___026___02472___z-584-9-3
Average 96 stars, based on 1486 article reviews
Dna, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+intercalator/Cell-ID+Intercalator-Ir/10__1038_slash_s41590___026___02472___z-584-9-3
Average 96 stars, based on 1486 article reviews
dna - by Bioz Stars,
2026-09
96/100 stars
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Staining:Article Title: IL-17A-producing γδ T cells and classical monocytes are associated with a rapid alloimmune response following vascularized composite allotransplantation in mice Article Snippet: .. After careful washing and recounting of the cells, the cells were stained with a Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: Next, cells were permeabilized with ice-cold methanol (Sigma), on ice for 10 min. Membrane permeabilized cells were washed twice with CSM, and all isotope-labeled antibodies against intracellular antigens were pre-mixed and filtered before staining in the same fashion as surface antibodies. .. After intracellular antibody staining, cells were washed once with CSM and then re-suspended in Article Title: Assessment of Imaging Mass Cytometry (IMC) as a Tool to Characterize Circulating Tumor Cells (CTCs) in Preclinical Mouse Models Article Snippet: .. Slides were blocked with 3% BSA in PBS for 1 h and stained with a mixture of metal-labeled antibodies at different dilutions (Table S1) overnight at 4 0 C. Slides were washed with PBS and counterstained with Cell‐IDTM Ir191/193 genomic Article Title: Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS–chemokine–myeloid axis Article Snippet: Samples were subjected to heat-mediated epitope retrieval in tris-EDTA (pH 9) at 98°C for 20 min. Tissues were then blocked with 3% BSA (A7906, Sigma-Aldrich) for 30 min at RT and stained overnight with the IMC Ab cocktail detailed in table S3 at 4°C. .. The following day, excess Ab was washed off with tris-buffered saline-Tween, nuclei were stained with Article Title: Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS-chemokine-myeloid axis. Article Snippet: Samples were subjected to heat- mediated epitope retrieval in tris- EDTA (pH 9) at 98°C for 20 min. Tissues were then blocked with 3% BSA (A7906, Sigma- Aldrich) for 30 min at RT and stained overnight with the IMC Ab cocktail detailed in table S3 at 4°C. .. The following day, excess Ab was washed off with tris- buffered saline- Tween, nuclei were stained with Incubation:Article Title: IL-17A-producing γδ T cells and classical monocytes are associated with a rapid alloimmune response following vascularized composite allotransplantation in mice Article Snippet: .. After careful washing and recounting of the cells, the cells were stained with a Article Title: Method for treating cancer Article Snippet: After incubation, samples were washed once with CSM, permeabilized with 1×eBioscience Permeabilization Buffer (00-8333-56, Thermo Fisher Scientific) on ice for 10 minutes, and then incubated with metal-conjugated antibodies against intracellular molecules for 1 hour. .. Cells were washed once with 1×eBioscience Permeabilization Buffer and then incubated at room temperature for 20 minutes with an iridium-containing Article Title: c-JUN enhances CRISPR knockin anti-B7-H3 CAR T cell function in small cell lung cancer and thoracic SMARCA4-deficient undifferentiated tumors. Article Snippet: .. Finally, samples were resuspended in Article Title: c-JUN enhances CRISPR knockin anti-B7-H3 CAR T cell function in small cell lung cancer and thoracic SMARCA4-deficient undifferentiated tumors Article Snippet: .. Finally, samples were resuspended in Cytometry:Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: Next, cells were permeabilized with ice-cold methanol (Sigma), on ice for 10 min. Membrane permeabilized cells were washed twice with CSM, and all isotope-labeled antibodies against intracellular antigens were pre-mixed and filtered before staining in the same fashion as surface antibodies. .. After intracellular antibody staining, cells were washed once with CSM and then re-suspended in Saline:Article Title: Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS–chemokine–myeloid axis Article Snippet: Samples were subjected to heat-mediated epitope retrieval in tris-EDTA (pH 9) at 98°C for 20 min. Tissues were then blocked with 3% BSA (A7906, Sigma-Aldrich) for 30 min at RT and stained overnight with the IMC Ab cocktail detailed in table S3 at 4°C. .. The following day, excess Ab was washed off with tris-buffered saline-Tween, nuclei were stained with Article Title: Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS-chemokine-myeloid axis. Article Snippet: Samples were subjected to heat- mediated epitope retrieval in tris- EDTA (pH 9) at 98°C for 20 min. Tissues were then blocked with 3% BSA (A7906, Sigma- Aldrich) for 30 min at RT and stained overnight with the IMC Ab cocktail detailed in table S3 at 4°C. .. The following day, excess Ab was washed off with tris- buffered saline- Tween, nuclei were stained with |